IL-6 antibody

Catalog No. ABIN6963871

This anti-IL-6 antibody is a Rat Monoclonal antibody detecting IL-6 in ELISA and ELISpot. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 5+ publications.

Quick Overview for IL-6 antibody (ABIN6963871)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: ELISA, ELISpot

Clone: 13A5

Product Details anti-IL-6 Antibody

Specificity: Native and recombinant IL-6

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-6 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-6 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-6

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963871, ABIN7448314 and ABIN6964024 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb ABIN6963872 and ABIN7448315 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-6 antibody (ABIN6963871)

Palizgir, Akhtari, Mahmoudi, Mostafaei, Rezaiemanesh, Shahram: "Curcumin reduces the expression of interleukin 1β and the production of interleukin 6 and tumor necrosis factor alpha by M1 macrophages from patients with Behcet's disease." in: Immunopharmacology and immunotoxicology, Vol. 40, Issue 4, pp. 297-302, (2018) (PubMed).

Sui, Hogg, Wang, Frey, Yu, Xia, Venzon, McKinnon, Smedley, Gathuka, Klinman, Keele, Langermann, Liu, Franchini, Berzofsky: "Vaccine-induced myeloid cell population dampens protective immunity to SIV." in: The Journal of clinical investigation, Vol. 124, Issue 6, pp. 2538-49, (2014) (PubMed).

Gille-Johnson, Smedman, Gudmundsdotter, Somell, Nihlmark, Paulie, Andersson, Gårdlund: "Circulating monocytes are not the major source of plasma cytokines in patients with sepsis." in: Shock (Augusta, Ga.), Vol. 38, Issue 6, pp. 577-83, (2013) (PubMed).

Feliu, Walter, Montañez, Kunzmann, Hult, Nyström, Malkoch, Fadeel: "Stability and biocompatibility of a library of polyester dendrimers in comparison to polyamidoamine dendrimers." in: Biomaterials, Vol. 33, Issue 7, pp. 1970-81, (2012) (PubMed).

Snyder-Cappione, Tincati, Eccles-James, Cappione, Ndhlovu, Koth, Nixon: "A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-α and MIP1-β, a lack of IL-17, and a Th1-bias in males." in: PLoS ONE, Vol. 5, Issue 11, pp. e15412, (2011) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Gene ID: 3569

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome