IL-6 antibody (Biotin)

Catalog No. ABIN6963872

This Rat Monoclonal antibody specifically detects IL-6 in ELISA and ELISpot. It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 4+ publications.

Quick Overview for IL-6 antibody (Biotin) (ABIN6963872)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 39C3

Product Details anti-IL-6 Antibody (Biotin)

Specificity: Native and recombinant IL-6

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-6 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-6 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-6

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963872 and ABIN7448315 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963871, ABIN7448314 and ABIN6964024 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-6 antibody (Biotin) (ABIN6963872)

Järver, Dondalska, Poux, Sandberg, Bergenstråhle, Sköld, Dereuddre-Bosquet, Martinon, Pålsson, Zaghloul, Brodin, Sander, Lennox, Behlke, El-Andaloussi, Lehtiö, Lundeberg, LeGrand, Spetz: "Single-Stranded Nucleic Acids Regulate TLR3/4/7 Activation through Interference with Clathrin-Mediated Endocytosis." in: Scientific reports, Vol. 8, Issue 1, pp. 15841, (2019) (PubMed).

Zhenjiang, Rao, Luo, Valentini, von Landenberg, Meng, Sinclair, Hoffmann, Karbach, Altmannsberger, Jäger, Peredo, Dodoo, Maeurer: "Cytokine Networks and Survivin Peptide-Specific Cellular Immune Responses Predict Improved Survival in Patients With Glioblastoma Multiforme." in: EBioMedicine, Vol. 33, pp. 49-56, (2018) (PubMed).

Phuah, Wong, Gideon, Maiello, Coleman, Hendricks, Ruden, Cirrincione, Chan, Lin, Flynn: "Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques." in: Infection and immunity, Vol. 84, Issue 5, pp. 1301-1311, (2017) (PubMed).

Min, Wang, Yuan, Kuang, Zhao: "Effects of repeated tuberculin skin testings on immune responses in experimental monkeys." in: Journal of animal science and technology, Vol. 56, pp. 32, (2015) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Gene ID: 3569

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome