Perforin 1 antibody (Biotin)

Catalog No. ABIN6963874

This anti-Perforin 1 antibody is a Mouse Monoclonal antibody detecting Perforin 1 in WB, ELISA, FACS, ELISpot and ISt. Suitable for Human, Pig and Non-Human Primate. This Primary Antibody has been cited in 5+ publications.

Quick Overview for Perforin 1 antibody (Biotin) (ABIN6963874)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human, Pig, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), ELISpot, Immunostaining (ISt)

Clone: Pf-344

Product Details anti-Perforin 1 Antibody (Biotin)

Specificity: Native Perforin

Cross-Reactivity (Details): The antibody also cross-reacts with Perforin from pig.

Characteristics: This monoclonal antibody enables specific detection of human perforin in immunoassays such as ELISpot, FluoroSpot, ELISA, flow cytometry, and Western blot. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Native human perforin

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963874, ABIN7489142, ABIN6963875, ABIN6963876 and ABIN6963877 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs . ABIN6963874, ABIN7489142, ABIN6963875, ABIN6963876 and ABIN6963877 is also suitable for immunocytochemistry, flow cytometry and Western Blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Perforin 1 antibody (Biotin) (ABIN6963874)

Rout: "Enhanced Th1/Th17 Functions of CD161+ CD8+ T Cells in Mucosal Tissues of Rhesus Macaques." in: PLoS ONE, Vol. 11, Issue 6, pp. e0157407, (2017) (PubMed).

Kim, Saide, Farrell, Faulkner, Tailor, Ogese, Daly, Pirmohamed, Park, Naisbitt: "Characterization of amoxicillin- and clavulanic acid-specific T cells in patients with amoxicillin-clavulanate-induced liver injury." in: Hepatology (Baltimore, Md.), Vol. 62, Issue 3, pp. 887-99, (2015) (PubMed).

Gudmundsdotter, Wahren, Haller, Boberg, Edbäck, Bernasconi, Buttò, Gaines, Imami, Gotch, Lori, Lisziewicz, Sandström, Hejdeman: "Amplified antigen-specific immune responses in HIV-1 infected individuals in a double blind DNA immunization and therapy interruption trial." in: Vaccine, Vol. 29, Issue 33, pp. 5558-66, (2011) (PubMed).

Chung, Hung, Yang, Su, Huang, Wei, Chin, Chiou, Chu, Ho, Yang, Lu, Wu, Liao, Chen: "Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis." in: Nature medicine, Vol. 14, Issue 12, pp. 1343-50, (2008) (PubMed).

Adhikary, Behrends, Moosmann, Witter, Bornkamm, Mautner: "Control of Epstein-Barr virus infection in vitro by T helper cells specific for virion glycoproteins." in: The Journal of experimental medicine, Vol. 203, Issue 4, pp. 995-1006, (2006) (PubMed).

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Gene ID: 5551, 396595

Pathways: Apoptosis, Caspase Cascade in Apoptosis