Perforin 1 antibody (FITC)

Catalog No. ABIN6963875

This Mouse Monoclonal antibody specifically detects Perforin 1 in FACS. It exhibits reactivity toward Human, Pig and Non-Human Primate.

Quick Overview for Perforin 1 antibody (FITC) (ABIN6963875)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human, Pig, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: Pf-344

Product Details anti-Perforin 1 Antibody (FITC)

Specificity: Native Perforin.

Cross-Reactivity (Details): The antibody also cross-reacts with Perforin from pig.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Native human Perforin

Isotype: IgG1

Application Details

Application Notes: Detection of Perforin producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of Perforin by flow cytometry in viable human peripheral blood mononuclear cells (PBMC). Human PBMC were fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with Pf-344-FITC (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Gene ID: 5551, 396595

Pathways: Apoptosis, Caspase Cascade in Apoptosis