IL-13 antibody (Biotin)

Catalog No. ABIN6963879

The Rat Monoclonal anti-IL-13 antibody has been validated for ELISA and ELISpot. It is suitable to detect IL-13 in samples from Non-Human Primate. There are 4+ publications available.

Quick Overview for IL-13 antibody (Biotin) (ABIN6963879)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-13 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: IL13-3

Product Details anti-IL-13 Antibody (Biotin)

Specificity: Native and recombinant IL-13

Cross-Reactivity (Details): The monoclonal antibody reacts with human IL-13 and cross-reacts with IL-13 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of monkey IL-13 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-13

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963879 and ABIN7489144 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963878 and ABIN7489143 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-13 antibody (Biotin) (ABIN6963879)

Riccio, Pratt-Riccio, Bianco-Júnior, Sanchez, Totino, Carvalho, Daniel-Ribeiro: "Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research." in: Malaria journal, Vol. 14, pp. 166, (2016) (PubMed).

Sui, Hogg, Wang, Frey, Yu, Xia, Venzon, McKinnon, Smedley, Gathuka, Klinman, Keele, Langermann, Liu, Franchini, Berzofsky: "Vaccine-induced myeloid cell population dampens protective immunity to SIV." in: The Journal of clinical investigation, Vol. 124, Issue 6, pp. 2538-49, (2014) (PubMed).

Wang, Metcalf, Ribeiro, McClure, Kaur: "Th-1-type cytotoxic CD8+ T-lymphocyte responses to simian immunodeficiency virus (SIV) are a consistent feature of natural SIV infection in sooty mangabeys." in: Journal of virology, Vol. 80, Issue 6, pp. 2771-83, (2006) (PubMed).

Mäkitalo, Andersson, Areström, Karlén, Villinger, Ansari, Paulie, Thorstensson, Ahlborg: "ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques." in: Journal of immunological methods, Vol. 270, Issue 1, pp. 85-97, (2002) (PubMed).

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Gene ID: 3596

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport