NCOA3 antibody

Catalog No. ABIN6971371

The Rabbit Polyclonal anti-NCOA3 antibody (ABIN6971371) specifically detects NCOA3 in WB and ChIP. The antibody is reactive with Human samples.

Quick Overview for NCOA3 antibody (ABIN6971371)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-NCOA3 Antibody

Characteristics: AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFkB1 protein. AIB1 / SRC-3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: AIB1 / SRC-3

Molecular Weight: 160 kDa

NCBI Accession: NP_858045

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha