FTO antibody (N-Term)

Catalog No. ABIN6971672

Product Details anti-FTO Antibody

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: FTO (fat mass and obesity associated) is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. This protein has high activity towards single-stranded RNA containing 3-methyluracil and single-stranded DNA containing 3-methylthymine. FTO has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and has no activity towards 1-methylguanine or double-stranded DNA. FTO contributes to the regulation of the global metabolic rate, energy expenditure, energy homeostasis, regulation of body size and body fat accumulation. FTO has recently been shown to have the highest activity towards demethylation of 6-methyladeninine in single-stranded RNA suggesting that FTO is involved in processing of nuclear RNAs and may play an important role in RNA epigenetics. FTO antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the N-terminal region of human FTO.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: FTO (FTO Products)

Synonyms: AW743446 antibody, mKIAA1752 antibody, RGD1305121 antibody, FTO, alpha-ketoglutarate dependent dioxygenase antibody, fat mass and obesity associated antibody, fat mass and obesity associated L homeolog antibody, FTO antibody, Fto antibody, fto.L antibody

Molecular Weight: 60 kDa

NCBI Accession: NP_001073901