FOXA1 antibody (AA 7-103)

Catalog No. ABIN6972166

This anti-FOXA1 antibody is a Mouse Monoclonal antibody detecting FOXA1 in WB, IHC, ChIP and ChIP-seq. Suitable for Human and Mouse.

Quick Overview for FOXA1 antibody (AA 7-103) (ABIN6972166)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 2F83

Product Details anti-FOXA1 Antibody

Binding Specificity: AA 7-103

Characteristics: HNF-3a (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1a, HNF-1b, HNF-3a, HNF-3b, HNF-3g, HNF-4a, HNF-4g, HNF-6a and HNF-6b), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3a and HNF-3g have also been shown to be involved in gastrulation events such as body axis formation. HNF-3a / FOXA1 antibody (mAb) (Clone 2F83) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Protein G Chromatography

Immunogen: This HNF-3α / FOXA1 antibody was raised against a recombinant protein corresponding to amino acids 7-103 of rat HNF-3α /FOXA1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: HNF-3α / FOXA1

Molecular Weight: 50 kDa

NCBI Accession: NP_036874

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis