MYOD1 antibody

Catalog No. ABIN6972391

The Rat Monoclonal anti-MYOD1 antibody (Clone 5F11) (ABIN6972391) specifically detects MYOD1 in WB, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for MYOD1 antibody (ABIN6972391)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5F11

Product Details anti-MYOD1 Antibody

Characteristics: MyoD (Myogenic differentiation factor 1, Myf-3), is a transcription factor belonging to the myogenic factors family that contains a basic helix-loop-helix (bHLH) DNA-binding domain. MyoD is the central regulator in the signaling pathways that regulate muscle development. MyoD antibody (mAb) (Clone 5F11) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Protein G Chromatography

Immunogen: This MyoD antibody was raised against a recombinant protein corresponding to full-length mouse MyoD.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MyoD

Molecular Weight: 40 kDa

NCBI Accession: NP_034996

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development