RAR-alpha (AA 420-443) antibody

Catalog No. ABIN6972582

The Mouse Monoclonal anti-RAR-alpha antibody is suitable to detect RAR-alpha in samples from Human and Mouse. It has been validated for IHC, SSA and WB.

Quick Overview for RAR-alpha (AA 420-443) antibody (ABIN6972582)

Target: RAR-alpha

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Supershift Assay (SSA), Western Blotting (WB)

Clone: 9alpha-9A6

Product Details

Binding Specificity: AA 420-443

Characteristics: Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-a, RAR-b and RAR-g. RAR-a (Nuclear receptor subfamily 1 group B member 1, NR1B1) interacts with NCoA3, NCoA6, NCoA7 and MLL to activate transcription of target genes. Chromosomal aberrations of RAR-a are sometimes involved in Promyelocytic leukemia. RAR-alpha antibody (mAb) (Clone 9a-9A6) was raised in a Mouse host. It has been validated for use in Immunohistochemistry, Supershift and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Protein A Chromatography

Immunogen: This RAR-a antibody was raised against a recombinant protein corresponding to amino acids 420-443 (the F region) of human RARa.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 60 mM Sodium Citrate, 105 mM Tris-HCl, 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details

Target: RAR-alpha

Alternative Name: RAR-α

Molecular Weight: 52 kDa

NCBI Accession: NP_001138773