RAR-alpha (AA 428-441) antibody
Reactivity: Human
IHC, SSA, WB
Host: Mouse
Monoclonal
4gamma-7A11
unconjugated
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- Target
- RAR-alpha
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Binding Specificity
- AA 428-441
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Supershift Assay (SSA), Western Blotting (WB)
- Characteristics
- Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-a, RAR-b and RAR-g. RAR-g has two splice isoforms. RAR-g heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with co-repressor protein. Binding of agonist ligands to RAR results in dissociation of co-repressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein. RAR-gamma antibody (mAb) (Clone 4g-7A11) was raised in a Mouse host. It has been validated for use in Immunohistochemistry, Supershift and Western blot, it has been shown to react with Human samples.
- Purification
- Protein G Chromatography
- Immunogen
- This RAR-γ antibody was raised against a recombinant protein corresponding to amino acids 428-441 (F-region) of human RARγ.
- Clone
- 4gamma-7A11
- Isotype
- IgG1 kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Target
- RAR-alpha
- Alternative Name
- RAR-γ
- Molecular Weight
- 52 kDa
- NCBI Accession
- NP_000957
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