RAR-alpha (AA 428-441) antibody

Catalog No. ABIN6972585

The Mouse Monoclonal anti-RAR-alpha antibody is suitable to detect RAR-alpha in samples from Human. It has been validated for IHC, SSA and WB.

Quick Overview for RAR-alpha (AA 428-441) antibody (ABIN6972585)

Target: RAR-alpha

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Supershift Assay (SSA), Western Blotting (WB)

Clone: 4gamma-7A11

Product Details

Binding Specificity: AA 428-441

Characteristics: Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-a, RAR-b and RAR-g. RAR-g has two splice isoforms. RAR-g heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with co-repressor protein. Binding of agonist ligands to RAR results in dissociation of co-repressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein. RAR-gamma antibody (mAb) (Clone 4g-7A11) was raised in a Mouse host. It has been validated for use in Immunohistochemistry, Supershift and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This RAR-γ antibody was raised against a recombinant protein corresponding to amino acids 428-441 (F-region) of human RARγ.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details

Target: RAR-alpha

Alternative Name: RAR-γ

Molecular Weight: 52 kDa

NCBI Accession: NP_000957