TDG antibody

Catalog No. ABIN6972851

This Rabbit Polyclonal antibody specifically detects TDG in WB. It exhibits reactivity toward Mouse.

Quick Overview for TDG antibody (ABIN6972851)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TDG Antibody

Characteristics: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG / Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway. TDG / Thymine-DNA glycosylase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Mouse samples.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a full-length recombinant protein corresponding to mouse thymine DNA glycosylase (TDG).

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG / Thymine-DNA glycosylase

Molecular Weight: 55 kDa

NCBI Accession: NP_766140

Pathways: DNA Damage Repair, Chromatin Binding