VPS4B antibody (AA 301-400) (AbBy Fluor® 680)

Catalog No. ABIN6980738

The Rabbit Polyclonal anti-VPS4B antibody is suitable to detect VPS4B in samples from Human, Mouse and Fish. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for VPS4B antibody (AA 301-400) (AbBy Fluor® 680) (ABIN6980738)

Target: VPS4B (vps4b) (Vacuolar Protein Sorting 4 Homolog B (vps4b))

Reactivity: Human, Mouse, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS4B antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-VPS4B Antibody (AbBy Fluor® 680)

Binding Specificity: AA 301-400

Cross-Reactivity: Fish, Human, Mouse

Predicted Reactivity: Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VPS4B

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for VPS4B

Target: VPS4B (vps4b) (Vacuolar Protein Sorting 4 Homolog B (vps4b))

Alternative Name: VPS4B

Background:

Synonyms: 8030489C12Rik, Cell migration inducing 1, Cell migration-inducing gene 1 protein, MGC116271, MIG1, Protein SKD1, Skd1, SKD1B, Suppressor of K(+) transport growth defect 1, Suppressor of K+ transport defect 1, Vacuolar protein sorting 4 homolog B (S. cerevisiae), Vacuolar protein sorting 4 homolog B, Vacuolar protein sorting 4b, Vacuolar protein sorting-associated protein 4B, Vacuolar protein sorting-associating protein 4B, VPS4 2, VPS42, Vps4b, VPS4B_HUMAN.

Background: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

Gene ID: 9525

UniProt: O75351

Pathways: Microtubule Dynamics, CXCR4-mediated Signaling Events