TUBB antibody (AA 61-160) (Biotin)

Catalog No. ABIN6982769

The Rabbit Polyclonal anti-TUBB antibody has been validated for WB, IHC (p) and IHC (fro). It is suitable to detect TUBB in samples from Human, Mouse and Rat.

Quick Overview for TUBB antibody (AA 61-160) (Biotin) (ABIN6982769)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TUBB Antibody (Biotin)

Binding Specificity: AA 61-160

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Beta tubulin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: Beta tubulin

Background:

Synonyms: Beta 4 tubulin, Tubulin-beta, Tubulin beta, Beta 5 tubulin, BetaTubulin, Beta-Tubulin, dJ40E16.7, TUBB, TUBB2, TUBB2A, TUBB5, tubulin beta 2A, Tubulin beta chain, Tubulin beta-5 chain, TUBB4A, TUBB4, Tubulin 5 beta, Tubulin beta-4 chain, TBB4A_HUMAN, Tubulin beta-4A chain.

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Gene ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase