MED12 antibody (AA 251-350) (Cy3)

Catalog No. ABIN6983572

This Rabbit Polyclonal antibody specifically detects MED12 in WB, IF (cc), IF (p). It exhibits reactivity toward Mouse, Rat.

Quick Overview for MED12 antibody (AA 251-350) (Cy3) (ABIN6983572)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MED12 Antibody (Cy3)

Binding Specificity: AA 251-350

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MED12/Trap230

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: MED12/Trap230

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 12, MED12, Activator-recruited cofactor 240 kDa component, ARC240, CAG repeat protein 45 Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trap230, Trinucleotide repeat-containing gene 11 protein, MED12, ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.

Gene ID: 9968

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation