DEPTOR antibody (AA 151-250) (Cy3)

Catalog No. ABIN6983879

The Rabbit Polyclonal anti-DEPTOR antibody is suitable to detect DEPTOR in samples from Human and Mouse. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for DEPTOR antibody (AA 151-250) (Cy3) (ABIN6983879)

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DEPTOR Antibody (Cy3)

Binding Specificity: AA 151-250

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DEPTOR

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: DEPTOR

Background:

Synonyms: DEP domain containing 6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPDC6, DEPTOR, DPTOR_HUMAN.

Background: DEPTOR (DEP domain containing MTOR-interacting protein), also known as DEP.6 or DEPDC6 (DEP domain-containing protein 6), is a 409 amino acid protein that negatively regulates mTORC1 and mTORC2 pathways. DEPTOR interacts with FRAP via its PDZ domain, and undergoes post-translational phosphorylation. Containing two DEP domains and one PDZ (DHR) domain, DEPTOR is encoded by a gene that maps to human chromosome 8q24.12. Chromosome 8 consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Gene ID: 64798

UniProt: Q8TB45

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect