Bcl-2 antibody (AA 51-150) (HRP)

Catalog No. ABIN6988060

This Mouse Monoclonal antibody specifically detects Bcl-2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Rabbit, Sheep, Dog, Cow, Guinea Pig and Horse.

Quick Overview for Bcl-2 antibody (AA 51-150) (HRP) (ABIN6988060)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Sheep, Dog, Cow, Guinea Pig, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is conjugated to HRP

Application: Western Blotting (WB)

Clone: 4D10

Product Details anti-Bcl-2 Antibody (HRP)

Binding Specificity: AA 51-150

Cross-Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background:

Synonyms: Apoptosis regulator Bcl 2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, B cell lymphoma 2, Bcl 2, Bcl-2, Bcl2, BCL2 protein, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, BCL2_HUMAN.

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14,18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling