DDX11 antibody

Catalog No. ABIN6989105

The Rabbit Polyclonal anti-DDX11 antibody is suitable to detect DDX11 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and ICC.

Quick Overview for DDX11 antibody (ABIN6989105)

Target: DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-DDX11 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KRG2/DDX11

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DDX11

Target: DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))

Alternative Name: KRG2/DDX11

Background:

Synonyms: CHL1, CHL1 related helicase gene 1, CHL1-like helicase homolog, CHL1-related protein 1, CHLR1, Ddx11, DDX11_HUMAN, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 11, DEAD/H box protein 11, hCHLR1, Keratinocyte growth factor regulated gene 2 protein, Keratinocyte growth factor-regulated gene 2 protein, KRG 2, KRG-2, KRG2, Probable ATP dependent RNA helicase DDX11, Probable ATP-dependent RNA helicase DDX11.

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Gene ID: 1663

UniProt: Q96FC9

Pathways: ER-Nucleus Signaling