GPER antibody (AA 21-120)

Catalog No. ABIN6989279

The Rabbit Polyclonal anti-GPER antibody is suitable to detect GPER in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IF (cc), IF (p), IHC (p), ICC and IHC (fro).

Quick Overview for GPER antibody (AA 21-120) (ABIN6989279)

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPER antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GPER Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GPR30

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Alternative Name: GPR30,

Background:

Synonyms: G Protein Coupled Receptor 30, G-protein coupled receptor 30, G-protein coupled estrogen receptor 1, Membrane estrogen receptor, mER, Chemokine receptor-like 2, IL8-related receptor DRY12, Flow-induced endothelial G-protein coupled receptor 1, FEG-1, Lymphocyte-derived G-protein coupled receptor, LYGPR, GPCR-BR, CEPR, CMKRL2, DRY12, GPER.

Background: G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GRP30 is a member of this family and is an orphan receptor. GPR30 expression has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. In brain, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. However, unlike other known G protein-coupled receptors, GPR30 localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives.

Gene ID: 2852

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process