MYD88 antibody (AA 176-280)

Catalog No. ABIN6990183

The Mouse Monoclonal anti-MYD88 antibody (Clone 2E9C2) (ABIN6990183) specifically detects MYD88 in WB, ELISA, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for MYD88 antibody (AA 176-280) (ABIN6990183)

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYD88 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2E9C2

Product Details anti-MYD88 Antibody

Binding Specificity: AA 176-280

Purification: MyD88 Monoclonal Antibody is Protein A purified.

Immunogen: Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.

Isotype: IgG2b

Application Details

Application Notes: MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MyD88 Monoclonal Antibody is supplied in PBS containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: MyD88 monoclonal antibody can be stored at 2-8°C, stable for one year.

Target Details for MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Alternative Name: MyD88

Background: MyD88 Monoclonal Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κ,B activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

Gene ID: 4615

UniProt: Q99836

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades