ORAI3 antibody (C-Term)

Catalog No. ABIN6990191

Product Details anti-ORAI3 Antibody

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Binding Specificity: C-Term

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORAI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Immunogen: A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

Clone: 2H2G9

Isotype: IgG2a

Application Details

Application Notes: ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: ORAI3 monoclonal antibody can be stored at -20°C, stable for one year.

Target Details for ORAI3

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Alternative Name: ORAI3 (ORAI3 Products)

Synonyms: ORAI3 antibody, TMEM142C antibody, 9930124N15 antibody, BC061259 antibody, Tmem142c antibody, RGD1306538 antibody, ORAI calcium release-activated calcium modulator 3 antibody, ORAI3 antibody, Orai3 antibody

Background: ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE, these may represent post-translationally modified ORAI 3.

Molecular Weight: Predicted: 32 kDa

Observed: 32 kDa

Gene ID: 93129

UniProt: Q9BRQ5