IRAK1 antibody (C-Term)

Catalog No. ABIN6990232

This Rabbit Polyclonal antibody specifically detects IRAK1 in WB, ELISA, IF, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IRAK1 antibody (C-Term) (ABIN6990232)

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-IRAK1 Antibody

Binding Specificity: C-Term

Specificity: At least four isoforms of IRAK are known to exist, this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.

Purification: IRAK Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK.

Isotype: IgG

Application Details

Application Notes: WB: 1-4 μ,g/mL, IF: 20 μ,g/mL, ICC: 10 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRAK Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRAK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: IRAK

Background: IRAK Antibody: Nuclear factor kappa B (NF-κ,B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ,B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κ,B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Molecular Weight: Predicted: 77kD

Observed: 77 kD kDa

Gene ID: 3654

UniProt: P51617

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades