SIRPA antibody (C-Term)

Catalog No. ABIN6990246

The Rabbit Polyclonal anti-SIRPA antibody (ABIN6990246) specifically detects SIRPA in WB, ELISA and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIRPA antibody (C-Term) (ABIN6990246)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-SIRPA Antibody

Binding Specificity: C-Term

Specificity: Recognizes SIRP alpha 1, 2 and 3.

Purification: SIRP alpha Antibody is affinity chromatography purified via peptide column.

Immunogen: SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1. The immunogen is located within the last 50 amino acids of SIRP alpha.

Isotype: IgG

Application Details

Application Notes: SIRP alpha antibody can be used for Western blot at 0.5 - 1 μ,g/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SIRP alpha Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SIRP alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: SIRP alpha

Background: SIRP alpha Antibody: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

Molecular Weight: 75 - 110 kDa

Gene ID: 140885

NCBI Accession: NP_542970

UniProt: P78324