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DFFA antibody (C-Term)

DFFA Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6990254
  • Target See all DFFA Antibodies
    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
    Binding Specificity
    • 15
    • 13
    • 9
    • 8
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 79
    • 31
    • 21
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 78
    • 10
    • 1
    Rabbit
    Clonality
    • 80
    • 9
    Polyclonal
    Conjugate
    • 59
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DFFA antibody is un-conjugated
    Application
    • 79
    • 30
    • 15
    • 14
    • 13
    • 13
    • 12
    • 10
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Purification
    DFF45 Antibody is Antibody is DEAE purified.
    Immunogen
    DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.
    Isotype
    IgG
    Top Product
    Discover our top product DFFA Primary Antibody
  • Application Notes
    DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μ,g/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    DFF45 Antibody is supplied in PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,4 °C
    Storage Comment
    DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
    Alternative Name
    DFF45 (DFFA Products)
    Synonyms
    DFF-45 antibody, DFF1 antibody, ICAD antibody, A330085O09Rik antibody, DFF35 antibody, Dff45 antibody, ICAD-L antibody, ICAD-S antibody, DFFA antibody, zgc:92229 antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha polypeptide antibody, DFFA antibody, Dffa antibody, dffa antibody
    Background
    DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
    Molecular Weight
    45 kDa
    Gene ID
    1676
    NCBI Accession
    NP_004392
    UniProt
    O00273
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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