TBK1 antibody (C-Term)

Catalog No. ABIN6990357

This anti-TBK1 antibody is a Rabbit Polyclonal antibody detecting TBK1 in WB, ELISA and ICC. Suitable for Human and Mouse.

Quick Overview for TBK1 antibody (C-Term) (ABIN6990357)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-TBK1 Antibody

Binding Specificity: C-Term

Specificity: No cross response to IKKa, IKKb, IKKy, or IKKe.

Purification: NAK Antibody is affinity chromatography purified via peptide column.

Immunogen: NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1. The immunogen is located within the last 50 amino acids of NAK.

Isotype: IgG

Application Details

Application Notes: NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.

Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NAK Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NAK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Alternative Name: NAK

Background: NAK Antibody: Nuclear factor kappa B (NF-κ,B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ,B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ,B is associated with Iκ,B proteins in the cell cytoplasm, which inhibit NF-κ,B activity. Phosphorylation of I-kappaB by Iκ,B kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κ,B. The IKK complex contains IKKα, IKKβ, and IKKγ,. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κ,B-activating kinase), and T2K. NAK/TBK1 activates IKKβ, through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κ,B activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

Molecular Weight: 84 kDa

Gene ID: 29110

NCBI Accession: NP_037386

UniProt: Q9UHD2

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome