Western Blotting (WB), ELISA, Immunofluorescence (IF)
Purification
IL-21 Receptor Antibody is affinity chromatography purified via peptide column.
Immunogen
IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 20 to 32 of human IL-21 receptor precursor. The immunogen is located within the first 50 amino acids of IL-21 Receptor.
IL21R
Reactivity: Human
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1 μ,g/mL. An approximately 60 kDa band can be detected. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
IL-21 Receptor Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
IL-21 Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IL-21 Receptor Antibody: A novel cytokine related to IL2 and IL15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.