KAP1 antibody (C-Term)

Catalog No. ABIN6990391

This Rabbit Polyclonal antibody specifically detects KAP1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KAP1 antibody (C-Term) (ABIN6990391)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KAP1 Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms of TRIM28 are known to exist, this antibody will detect all three isoforms

Purification: TRIM28 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal TRIM28 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TRIM28. The immunogen is located within the last 50 amino acids of TRIM28.

Isotype: IgG

Application Details

Application Notes: TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM28 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28

Background: TRIM28 Antibody: TRIM28, also known as KAP-1, is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily and contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TRIM28 is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TRIM28 acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TRIM28 associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.

Molecular Weight: Predicted: 92 kDa

Gene ID: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus