APOBEC3G antibody (N-Term)

Catalog No. ABIN6990450

This anti-APOBEC3G antibody is a Rabbit Polyclonal antibody detecting APOBEC3G in WB, ELISA and IHC (p). Suitable for Human.

Quick Overview for APOBEC3G antibody (N-Term) (ABIN6990450)

Target: APOBEC3G (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3G antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOBEC3G Antibody

Binding Specificity: N-Term

Specificity: APOBEC3G antibody will also detect the APOBEC3F isoform.

Purification: APOBEC3G Antibody is affinity chromatography purified via peptide column.

Immunogen: APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G.

Isotype: IgG

Application Details

Application Notes: APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APOBEC3G Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APOBEC3G antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for APOBEC3G

Target: APOBEC3G (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G))

Alternative Name: APOBEC3G

Background: APOBEC3G Antibody: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5α, which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G, a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

Gene ID: 60489

NCBI Accession: NP_068594

UniProt: Q9HC16