NAIP antibody (C-Term)

Catalog No. ABIN6990465

The Rabbit Polyclonal anti-NAIP antibody (ABIN6990465) specifically detects NAIP in WB, ELISA, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for NAIP antibody (C-Term) (ABIN6990465)

Target: NAIP (NLR Family, Apoptosis Inhibitory Protein (NAIP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NAIP Antibody

Binding Specificity: AA 1200-1250, C-Term

Specificity: Minor, lower molecular weight bands may represent alternately spliced forms.

Purification: NAIP Antibody is affinity chromatography purified via peptide column.

Immunogen: NAIP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP. The immunogen is located within amino acids 1200 - 1250 of NAIP.

Isotype: IgG

Application Details

Application Notes: NAIP antibody can be used for the detection of NAIP by Western blot at 0.5 to 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NAIP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NAIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NAIP

Target: NAIP (NLR Family, Apoptosis Inhibitory Protein (NAIP))

Alternative Name: NAIP

Background: NAIP Antibody: Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR), these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules. Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.

Molecular Weight: Predicted: 137, 154 kDa

Observed: 155 kDa

Gene ID: 4671

UniProt: Q13075

Pathways: Apoptosis, Inflammasome