Caspase 1 antibody (AA 200-250)

Catalog No. ABIN6990519

This anti-Caspase 1 antibody is a Rabbit Polyclonal antibody detecting Caspase 1 in WB, ELISA, IF and IHC (p). Suitable for Human.

Quick Overview for Caspase 1 antibody (AA 200-250) (ABIN6990519)

Target: Caspase 1 (CASP1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 1 Antibody

Binding Specificity: AA 200-250

Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.

Purification: Caspase-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-1 antibody was raised against a 15 amino acid synthetic peptide from near the middle of human Caspase-1. The immunogen is located within amino acids 200 - 250 of Caspase-1.

Isotype: IgG

Application Details

Application Notes: Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Caspase-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Background: Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β, and IL-18. Caspase-1 was initially identified as an IL-1β,-converting enzyme, later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Gene ID: 834

NCBI Accession: NP_150634

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome