LAMP1 antibody (AA 230-280)

Catalog No. ABIN6990580

This Rabbit Polyclonal antibody specifically detects LAMP1 in WB, IF, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LAMP1 antibody (AA 230-280) (ABIN6990580)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LAMP1 Antibody

Binding Specificity: AA 230-280

Specificity: LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE.

Purification: LAMP-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: LAMP-1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human LAMP-1. The immunogen is located within amino acids 230 - 280 of LAMP-1.

Isotype: IgG

Application Details

Application Notes: LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LAMP-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LAMP-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: LAMP-1

Background: LAMP-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.

Molecular Weight: Predicted: 46 kDa

Observed: 95 kDa

Gene ID: 3916

NCBI Accession: NP_005552

UniProt: P11279

Pathways: Autophagy