DIABLO antibody (C-Term)

Catalog No. ABIN6990637

This Rabbit Polyclonal antibody specifically detects DIABLO in WB, ELISA, IF, IP and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DIABLO antibody (C-Term) (ABIN6990637)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DIABLO Antibody

Binding Specificity: C-Term

Purification: Smac Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-Smac antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Smac. The immunogen is located within the last 50 amino acids of Smac.

Isotype: IgG

Application Details

Application Notes: WB: 1 μ,g/mL, IHC: 5 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Smac Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Smac antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: Smac

Background: Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

Molecular Weight: Predicted: 27kD

Observed: 20 kD kDa

Gene ID: 66593

NCBI Accession: NP_063940

UniProt: Q9JIQ3

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity