Nicastrin antibody (AA 370-420)

Catalog No. ABIN6990692

The Rabbit Polyclonal anti-Nicastrin antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect Nicastrin in samples from Human, Mouse and Rat.

Quick Overview for Nicastrin antibody (AA 370-420) (ABIN6990692)

Target: Nicastrin (NCSTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicastrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nicastrin Antibody

Binding Specificity: AA 370-420

Purification: Nicastrin Antibody is affinity chromatography purified via peptide column.

Immunogen: Nicastrin antibody was raised against a 18 amino acid synthetic peptide from near the center of human Nicastrin. The immunogen is located within amino acids 370 - 420 of Nicastrin.

Isotype: IgG

Application Details

Application Notes: Nicastrin antibody can be used for detection of Nicastrin by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Nicastrin Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Nicastrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Nicastrin

Target: Nicastrin (NCSTN)

Alternative Name: Nicastrin

Background: Nicastrin Antibody: Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

Gene ID: 23385

NCBI Accession: NP_056146

UniProt: Q92542

Pathways: Notch Signaling, Neurotrophin Signaling Pathway