Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

IFIH1 antibody (AA 330-380)

This Rabbit Polyclonal antibody specifically detects IFIH1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN6990715

Quick Overview for IFIH1 antibody (AA 330-380) (ABIN6990715)

Target

See all IFIH1 Antibodies
IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity

  • 44
  • 13
  • 4
  • 2
  • 2
  • 2
  • 1
Human, Mouse

Host

  • 37
  • 8
  • 2
Rabbit

Clonality

  • 36
  • 11
Polyclonal

Conjugate

  • 41
  • 3
  • 2
  • 1
This IFIH1 antibody is un-conjugated

Application

  • 37
  • 24
  • 21
  • 11
  • 8
  • 7
  • 6
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 330-380

    Purification

    MDA5 Antibody is affinity chromatography purified via peptide column.

    Immunogen

    MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the center of human MDA5. The immunogen is located within amino acids 330 - 380 of MDA5.

    Isotype

    IgG
  • Application Notes

    MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    MDA5 Antibody is supplied in PBS containing 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C,4 °C

    Storage Comment

    MDA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

    Alternative Name

    MDA5

    Background

    MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κ,B. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

    Gene ID

    64135

    NCBI Accession

    NP_071451

    UniProt

    Q9BYX4

    Pathways

    Activation of Innate immune Response
You are here:
Chat with us!