IL-33 antibody (N-Term)

Catalog No. ABIN6990769

This Rabbit Polyclonal antibody specifically detects IL-33 in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for IL-33 antibody (N-Term) (ABIN6990769)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-IL-33 Antibody

Binding Specificity: AA 50-100, N-Term

Purification: IL-33 Antibody is affinity chromatography purified via peptide column.

Immunogen: IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human IL-33. The immunogen is located within amino acids 50 - 100 of IL-33.

Isotype: IgG

Application Details

Application Notes: IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IL-33 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IL-33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Background: IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α,/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κ,B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.

Gene ID: 90865

NCBI Accession: NP_254274

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response