KEAP1 antibody (C-Term)

Catalog No. ABIN6990770

The Rabbit Polyclonal anti-KEAP1 antibody has been validated for WB, ELISA and IF. It is suitable to detect KEAP1 in samples from Human, Mouse and Rat.

Quick Overview for KEAP1 antibody (C-Term) (ABIN6990770)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-KEAP1 Antibody

Binding Specificity: C-Term

Specificity: At least two isoforms of KEAP1 are known to exist.

Purification: KEAP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: KEAP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: KEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background: KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κ,B activity by targeting IKKβ, degradation. Mutation of the KEAP1 gene is found in lung cancer.

Molecular Weight: Predicted: 70kD

Observed: 65-70kD kDa

Gene ID: 9817

NCBI Accession: NP_987096

UniProt: Q14145

Pathways: Maintenance of Protein Location