MANF antibody (N-Term)

Catalog No. ABIN6990804

The Rabbit Polyclonal anti-MANF antibody (ABIN6990804) specifically detects MANF in WB, ELISA, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MANF antibody (N-Term) (ABIN6990804)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MANF Antibody

Binding Specificity: N-Term

Specificity: This antibody does not cross-react with CDNF.

Purification: MANF Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-MANF antibody was raised against a peptide corresponding to 12 amino acids near the amino terminus of human MANF. The immunogen is located within the first 50 amino acids of MANF.

Isotype: IgG

Application Details

Application Notes: WB: 0.125-2 μ,g/mL, IHC: 2.5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MANF Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MANF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF

Background: MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

Molecular Weight: Predicted: 20kD

Observed: 26 kD kDa

Gene ID: 7873

UniProt: P55145