GPAM antibody (C-Term)

Catalog No. ABIN6990909

Product Details anti-GPAM Antibody

Target: GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM))

Binding Specificity: AA 730-780, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least four isoforms of GPAT1 are known to exist, this antibody will detect only the two largest isoforms.

Purification: GPAT1 Antibody is affinity chromatography purified via peptide column.

Immunogen: GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1.

Isotype: IgG

Application Details

Application Notes: GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: GPAT1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: GPAT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for GPAM

Target: GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM))

Alternative Name: GPAT1 (GPAM Products)

Synonyms: Gpam antibody, GPAT antibody, GPAT1 antibody, glycerol-3-phosphate acyltransferase, mitochondrial antibody, GPAM antibody, gpam antibody, Gpam antibody

Background: GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis, steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.

Molecular Weight: Predicted: 87, 91 kDa

Observed: 92 kDa

Gene ID: 57678

NCBI Accession: NP_065969

UniProt: Q9HCL2

Pathways: Activated T Cell Proliferation