PARL antibody (N-Term)

Catalog No. ABIN6990962

Product Details anti-PARL Antibody

Target: PARL (Presenilin Associated, Rhomboid-Like (PARL))

Binding Specificity: AA 40-90, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: PARL Antibody is affinity chromatography purified via peptide column.

Immunogen: PARL antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL. The immunogen is located within amino acids 40 - 90 of PARL.

Isotype: IgG

Application Details

Application Notes: PARL antibody can be used for detection of PARL by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight of ~42 kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PARL Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PARL antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PARL

Target: PARL (Presenilin Associated, Rhomboid-Like (PARL))

Alternative Name: PARL (PARL Products)

Synonyms: PSARL antibody, PSARL1 antibody, PSENIP2 antibody, RHBDS1 antibody, D16Ertd607e antibody, PRO2207 antibody, Psarl antibody, parl antibody, zgc:112986 antibody, presenilin associated rhomboid like antibody, presenilin associated, rhomboid-like antibody, presenilin associated, rhomboid-like a antibody, PARL antibody, Parl antibody, parla antibody

Background: PARL Antibody: PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL's association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer's disease through its associations with presenilin-1 and -2. Multiple isoforms of PARL are known to exist.

Gene ID: 55486

UniProt: Q9H300

Pathways: Autophagy