ATG5 antibody (N-Term)

Catalog No. ABIN6991065

The Chicken Polyclonal anti-ATG5 antibody (ABIN6991065) specifically detects ATG5 in WB, ELISA and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for ATG5 antibody (N-Term) (ABIN6991065)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG5 Antibody

Binding Specificity: N-Term

Specificity: Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.

Purification: ATG5 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5. The immunogen is located within the first 50 amino acids of ATG5.

Isotype: IgY

Application Details

Application Notes: WB: 1-2 μ,g/mL, IHC-P: 2.5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ATG5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

Molecular Weight: Predicted: 32kD

Observed: 55kD (ATG5-ATG12 conjugation) kDa

Gene ID: 9474

UniProt: A9UGY9

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy