LIF antibody (AA 50-100)

Catalog No. ABIN6991509

The Rabbit Polyclonal anti-LIF antibody (ABIN6991509) specifically detects LIF in WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LIF antibody (AA 50-100) (ABIN6991509)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-LIF Antibody

Binding Specificity: AA 50-100

Specificity: At least two isoforms of LIF are known to exist.

Purification: LIF Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-LIF antibody was raised against a peptide corresponding to 16 amino acids near the center of human LIF. The immunogen is located within amino acids 50-100 of LIF.

Isotype: IgG

Application Details

Application Notes: WB: 1-4 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LIF Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LIF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

Molecular Weight: Predicted: 22kD

Observed: 26kD (Post-modifications: N-linked Glycosylations) kDa

Gene ID: 3976

NCBI Accession: NP_002300

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding