NUMB antibody (C-Term)

Catalog No. ABIN6991533

This anti-NUMB antibody is a Rabbit Polyclonal antibody detecting NUMB in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for NUMB antibody (C-Term) (ABIN6991533)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NUMB Antibody

Binding Specificity: C-Term

Specificity: At least four isoforms of NUMB are known to exist, this antibody will detect all of them.

Purification: NUMB Antibody is affinity chromatography purified via peptide column.

Immunogen: NUMB antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human NUMB. The immunogen is located within the last 50 amino acids of NUMB.

Isotype: IgG

Application Details

Application Notes: NUMB antibody can be used for detection of NUMB by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NUMB Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NUMB antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background: NUMB Antibody: NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

Gene ID: 8650

NCBI Accession: NP_1005743

UniProt: P49757

Pathways: Cell-Cell Junction Organization