DPAGT1 antibody (N-Term)

Catalog No. ABIN6991580

Product Details anti-DPAGT1 Antibody

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Binding Specificity: AA 30-80, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPAGT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least four isoforms of DPAGT1 are known to exist, this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.

Purification: DPAGT1 Antibody is affinity chromatography purified via peptide column.

Immunogen: DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1. The immunogen is located within amino acids 30 - 80 of DPAGT1.

Isotype: IgG

Application Details

Application Notes: DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DPAGT1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DPAGT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DPAGT1

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Alternative Name: DPAGT1 (DPAGT1 Products)

Synonyms: AU021132 antibody, Dpagt2 antibody, GPT antibody, Gnpta antibody, DPAGT2 antibody, G1PT antibody, GNPTA antibody, GTR2 antibody, ALG7 antibody, CDG-Ij antibody, CDG1J antibody, CMSTA2 antibody, D11S366 antibody, DGPT antibody, DPAGT antibody, UAGT antibody, UGAT antibody, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) antibody, dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 antibody, Dpagt1 antibody, DPAGT1 antibody

Background: DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.

Gene ID: 1798

NCBI Accession: NP_001373

UniProt: Q9H3H5