Nibrin antibody (N-Term)

Catalog No. ABIN6991690

This Rabbit Polyclonal antibody specifically detects Nibrin in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Nibrin antibody (N-Term) (ABIN6991690)

Target: Nibrin (NBN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nibrin Antibody

Binding Specificity: AA 50-100, N-Term

Specificity: At least three alternatively spliced transcript isoforms of NIBRIN are known to exist.

Purification: NIBRIN Antibody is affinity chromatography purified via peptide column.

Immunogen: NIBRIN antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NIBRIN. The immunogen is located within amino acids 50 - 100 of NIBRIN.

Isotype: IgG

Application Details

Application Notes: NIBRIN antibody can be used for detection of NIBRIN by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NIBRIN Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NIBRIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: NIBRIN

Background: NIBRIN Antibody: NIBRIN (NBN) is a member of the double-strand break repair complex MRE11/RAD50/NBN (MRN) which is involved in DNA double-strand break repair, DNA damage-induced checkpoint activation and plays a critical role in the maintenance of chromosome integrity. NIBRIN contains two modules found in cell cycle checkpoint proteins, a forkhead-associated domain adjacent to a breast cancer carboxy-terminal domain. Mutations in this gene are associated with Nijmegen breakage syndrome and maybe the cause of cancer predisposition and aplastic anemia.

Gene ID: 4683

NCBI Accession: NP_002476

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response