UNG antibody (AA 200-250)

Catalog No. ABIN6991910

The Rabbit Polyclonal anti-UNG antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect UNG in samples from Human and Mouse.

Quick Overview for UNG antibody (AA 200-250) (ABIN6991910)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UNG Antibody

Binding Specificity: AA 200-250

Purification: UNG1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-UNG1 antibody was raised against a peptide corresponding to 14 amino acids near the center of human UNG1. The immunogen is located within amino acids 200-250 of UNG1.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-4 μ,g/mL, IHC: 2 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UNG1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UNG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Alternative Name: UNG1

Background: UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.

Molecular Weight: Predicted: 34kD

Observed: 34 kD kDa

Gene ID: 7374

NCBI Accession: NP_003353

UniProt: P13051

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response