ARR3 antibody (C-Term)

Catalog No. ABIN6991953

The Rabbit Polyclonal anti-ARR3 antibody (ABIN6991953) specifically detects ARR3 in WB, ELISA, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ARR3 antibody (C-Term) (ABIN6991953)

Target: ARR3 (Arrestin 3, Retinal (X-Arrestin) (ARR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARR3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARR3 Antibody

Binding Specificity: C-Term

Specificity: ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.

Purification: ARR3 antibody is affinity chromatography purified via peptide column.

Immunogen: ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3. The immunogen is located within the last 50 amino acids of ARR3.

Isotype: IgG

Application Details

Application Notes: ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ARR3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ARR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ARR3

Target: ARR3 (Arrestin 3, Retinal (X-Arrestin) (ARR3))

Alternative Name: ARR3

Background: Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).

Molecular Weight: Predicted: 43 kDa

Observed: 47 kDa

Gene ID: 407

NCBI Accession: NP_004303

UniProt: P36575

Pathways: Phototransduction, Thromboxane A2 Receptor Signaling