UCP1 antibody (C-Term)

Catalog No. ABIN6991968

Product Details anti-UCP1 Antibody

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: UCP1 antibody is human, mouse and rat reactive.

Purification: UCP1 antibody is affinity chromatography purified via peptide column.

Immunogen: UCP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human UCP1 (GenBank accession. The immunogen is located within the last 50 amino acids of UCP1.

Isotype: IgG

Application Details

Application Notes: UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UCP1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UCP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1 (UCP1 Products)

Synonyms: ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1 antibody, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1 antibody, ATPUMP1 antibody, ATUCP1 antibody, UCP antibody, UCP1 antibody, UNCOUPLING PROTEIN 1 antibody, plant uncoupling mitochondrial protein 1 antibody, ucp antibody, slc25a7 antibody, SLC25A7 antibody, AI385626 antibody, Slc25a7 antibody, Ucp antibody, Ucpa antibody, Uncp antibody, plant uncoupling mitochondrial protein 1 antibody, uncoupling protein 1 antibody, uncoupling protein 1 L homeolog antibody, uncoupling protein 1 (mitochondrial, proton carrier) antibody, PUMP1 antibody, UCP1 antibody, ucp1.L antibody, ucp1 antibody, Ucp1 antibody

Background: The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP) (1). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells (1). UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat (1).

Molecular Weight: Predicted: 34 kDa

Observed: 35 kDa

Gene ID: 7350

NCBI Accession: NP_068605

UniProt: P25874

Pathways: Proton Transport, Brown Fat Cell Differentiation