HDAC2 antibody (C-Term)

Catalog No. ABIN6992035

This Rabbit Polyclonal antibody specifically detects HDAC2 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HDAC2 antibody (C-Term) (ABIN6992035)

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC2 Antibody

Binding Specificity: C-Term

Specificity: HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist, this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.

Purification: HDAC2 antibody is affinity chromatography purified via peptide column.

Immunogen: HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2. The immunogen is located within the last 50 amino acids of HDAC2.

Isotype: IgG

Application Details

Application Notes: HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: HDAC2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: HDAC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Alternative Name: HDAC2

Background: The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).

Molecular Weight: Predicted: 54 kDa

Observed: 52 kDa

Gene ID: 3066

NCBI Accession: NP_001518

UniProt: Q92769

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection