BAMBI antibody (AA 100-150)

Catalog No. ABIN6992047

This Rabbit Polyclonal antibody specifically detects BAMBI in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for BAMBI antibody (AA 100-150) (ABIN6992047)

Target: BAMBI (BMP and Activin Membrane-Bound Inhibitor Homolog (BAMBI))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAMBI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAMBI Antibody

Binding Specificity: AA 100-150

Specificity: BAMBI antibody is human, mouse and rat reactive.

Purification: BAMBI antibody is affinity chromatography purified via peptide column.

Immunogen: BAMBI antibody was raised against a 17 amino acid peptide near the center of human BAMBI. The immunogen is located within amino acids 100 - 150 of BAMBI.

Isotype: IgG

Application Details

Application Notes: BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BAMBI antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BAMBI antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for BAMBI

Target: BAMBI (BMP and Activin Membrane-Bound Inhibitor Homolog (BAMBI))

Alternative Name: BAMBI

Background: BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).

Molecular Weight: Predicted: 29 kDa

Observed: 26 kDa

Gene ID: 25805

NCBI Accession: NP_036474

UniProt: Q13145