Sestrin 2 antibody (AA 220-270)

Catalog No. ABIN6992081

Product Details anti-Sestrin 2 Antibody

Target: Sestrin 2 (SESN2)

Binding Specificity: AA 220-270

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sestrin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist, this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1.

Purification: SESTRIN2 antibody is affinity chromatography purified via peptide column.

Immunogen: SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2. The immunogen is located within amino acids 220 - 270 of SESTRIN2.

Isotype: IgG

Application Details

Application Notes: SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SESTRIN2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SESTRIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Sestrin 2

Target: Sestrin 2 (SESN2)

Alternative Name: SESTRIN2 (SESN2 Products)

Synonyms: HI95 antibody, SES2 antibody, SEST2 antibody, Ses2 antibody, MGC81239 antibody, SESN2 antibody, sestrin-2 antibody, hi95 antibody, ses2 antibody, sest2 antibody, Sestrin-2 antibody, RGD1566319 antibody, sestrin 2 antibody, sestrin 2 L homeolog antibody, SESN2 antibody, Sesn2 antibody, sesn2.L antibody, sesn2 antibody

Background: SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).

Molecular Weight: Predicted: 54 kDa

Observed: 53 kDa

Gene ID: 83667

NCBI Accession: NP_113647

UniProt: P58004